Journal: Clinical and Vaccine Immunology : CVI

Article Title: Characterization of Immune Responses to Capsid Protein p24 of Human Immunodeficiency Virus Type 1 and Implications for Detection ▿ † ‡

doi: 10.1128/CVI.00066-10

Figure Lengend Snippet: HIV-1 capsid protein, synthesized peptides, and anti-p24-reacting peptides. The HIV-1 capsid protein has 231 aa and consists of 11 helices (H1 to H11; gray cylinders) and two unique motifs, namely, the CypA binding loop and the major homology region (MHR). Nine overlapping peptides (p1 to p9; open squares), which extend from aa 10 to aa 227, were synthesized and used to identify the peptides that reacted with (indicated by black arrows) monoclonal anti-p24 antibodies (top).

Article Snippet: The differences in immune responses toward p24 peptides among the three groups were statistically significant ( P < 0.001; Fisher's exact test [SAS; SAS Institute, Inc., Cary, NC]).

Techniques: Synthesized, Binding Assay

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Characterization of Immune Responses to Capsid Protein p24 of Human Immunodeficiency Virus Type 1 and Implications for Detection ▿ † ‡

doi: 10.1128/CVI.00066-10

Figure Lengend Snippet: Characterization of monoclonal anti-HIV-1 p24 antibodies

Article Snippet: The differences in immune responses toward p24 peptides among the three groups were statistically significant ( P < 0.001; Fisher's exact test [SAS; SAS Institute, Inc., Cary, NC]).

Techniques: Recombinant

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Characterization of Immune Responses to Capsid Protein p24 of Human Immunodeficiency Virus Type 1 and Implications for Detection ▿ † ‡

doi: 10.1128/CVI.00066-10

Figure Lengend Snippet: Immune responses to HIV-1 p24 peptides and recombinant protein. Immune responses were quantified as described in Materials and Methods and were normalized by calculating the S/CO ratios relative to the level of HIV-1 recombinant p24 protein, which was arbitrarily set at 100%. The data were plotted in bar graphs, and error bars indicate standard deviations for three independent experiments. Numbers 1 to 9 and 10 on the x axis represent peptides p1 to p9 and p24 protein, respectively. (A) Polyclone-like response; (B, C, and D) monoclone-like response; (E and F) responses against conformational epitopes.

Article Snippet: The differences in immune responses toward p24 peptides among the three groups were statistically significant ( P < 0.001; Fisher's exact test [SAS; SAS Institute, Inc., Cary, NC]).

Techniques: Recombinant

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Characterization of Immune Responses to Capsid Protein p24 of Human Immunodeficiency Virus Type 1 and Implications for Detection ▿ † ‡

doi: 10.1128/CVI.00066-10

Figure Lengend Snippet: Lambda/kappa ratios in HIV-1-positive sera. (A) Lambda/kappa ratios for whole human Ig. Microplates were coated with goat anti-human Ig and incubated with dilutions of HIV-1-positive sera. Lambda/kappa ratios for antibodies were calculated as described in Materials and Methods. Identification numbers of the sera are shown on the x axis. (B) Lambda/kappa ratios for anti-p24 antibodies. Microplates were coated with recombinant HIV-1 p24 and incubated with dilutions of HIV-1-positive sera. The assay was done as described for panel A.

Article Snippet: The differences in immune responses toward p24 peptides among the three groups were statistically significant ( P < 0.001; Fisher's exact test [SAS; SAS Institute, Inc., Cary, NC]).

Techniques: Incubation, Recombinant